文章题目：Alterationsofthebilemicrobiomeinprimarysclerosingcholangitis
研究人员:timurliwinski
发表时间：.06
期刊名称：BMJ
研究单位：DepartmentofMedicine,UniversityMedicalCenterHamburg-Eppendorf,Hamburg,Germany
影响因子：23.
分区：1区
核心亮点
1、AddingtopreviouslyreportedchangesofthefaecalandcolonicmucosalmicrobiomeinPSC,ourstudyshowsthattheupperalimentarytractandthebileductsofPSCpatientsarelikewiseaffectedbymicrobialdysbiosis.除了之前报道的PSC中粪便和结肠黏膜微生物组的变化外，我们的研究表明，PSC患者的上消化道和胆管同样受到微生物失调的影响。
2、Recentnext-generationsequencing(NGS)studiesrevealedanalteredgutbacterialmicrobiomeinpatientswithPSC,bothatthefaecalandmucosallevel,whichwasdifferentfrombothhealthyindividualsandpatientswithUC.最近的下一代测序(NGS)研究显示，PSC患者的肠道菌群在粪便和粘膜水平上都发生了改变，这与健康人和UC患者都不同。
方法
Methods:PatientswithPSc(n=43)andcontrolswithoutsclerosingcholangitis(n=22)requiringendoscopicretrogradecholangiographywereincludedprospectively.leadingindicationsincontrolsweresporadiccholedocholithiasisandpapillaryadenoma.atotalofbiospecimenswerecollectedfromtheoralcavity,duodenalfluidandmucosaandductalbile.Microbiomesoftheupperalimentarytractandductalbilewereprofiledbysequencingthe16S-rrna-encodinggene(V1–V2).Bilefluidbileacid